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Open Access Green as soon as Postprint is submitted to ZB.
Isolation of functional RNA from plant tissues rich in phenolic compounds.
Anal. Biochem. 197, 91-95 (1991)
A method for the isolation of RNA from different tissues of trees (seedlings, saplings, and adult trees) is described. Using this procedure it is possible to remove large amounts of disturbing polyphenolic compounds from nucleic acids. The method involves an acetone treatment of the freeze-dried and powdered plant material, the use of high salt concentrations in the extraction buffer and an aqueous two-phase system. These steps were combined with the conventional phenol/chloroform extraction and CsCl centrifugation. The method has been successfully applied to the isolation and purification of RNA from pine (Pinus sylvestris L. and Pinus mugo Turr.), Norway spruce (Picea abies L.), and beech (Fagus sylvatica L.). The functional quality of RNA extracted by this procedure has been characterized by its uv spectrum, by agarose gel electrophoresis with ethidium bromide staining, Northern blot hybridization, and in vitro translation.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0003-2697
e-ISSN
1096-0309
Journal
Analytical Biochemistry
Quellenangaben
Volume: 197,
Issue: 1,
Pages: 91-95
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)