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Klose, U. ; Theirau, D. ; Veser, U. ; Schwartz, L.R.

Stimulation of DNA synthesis in carcinogen-induced diploid hepatocytes in vitro.

Carcinogenesis 11, 1479-1483 (1990)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Diploid hepatocytes induced by a combination of diethylnitrosamine and 2-acetylaminofluorene were isolated and separated from polyploid hepatocytes by centrifugal elutriation. The diploid and polyploid cell fractions were ~90% pure and contained between 1 and 1.5 x 107 cells. When kept in monolayer cultures both cell populations responded to the mitogenic effect of EGF and insulin. However, the percentage of labelled nuclei was higher in predominantly diploid compared to predominantly tetraploid hepatocyte cultures at all epidermal growth factor (EGF) concentrations used in this study. At 10 ng EGF/ml and 10 mU insulin/ml the labelling index was twice as high in the diploid liver cells. Further work is required to show the relevance of the stronger response of the diploid cell fraction to mitogens in the process of carcinogenesis.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 1990
HGF-reported in Year 0
ISSN (print) / ISBN 0143-3334
e-ISSN 1460-2180
Journal Carcinogenesis
Quellenangaben Volume: 11, Issue: 9, Pages: 1479-1483 Article Number: , Supplement: ,
Publisher Oxford University Press
Reviewing status Peer reviewed
Institute(s) Institut für Toxikologie und Biochemie
Scopus ID 11944253099
PubMed ID 2205406
Erfassungsdatum 1990-12-31
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