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Open Access Green as soon as Postprint is submitted to ZB.
Time-resolved fluorescence microscopy for measuring specific coenzymes in methanogenic bacteria.
Anal. Chim. Acta 163, 249-255 (1984)
Measurements of time-resolved photobleaching and nanosecond fluorescence decay from microscopic samples of methanogenic bacteria are reported. From cultures of Methanobacterium thermoautotrophicum and Methanosarcina barkeri, decay times of 1 ns and 3 ns were obtained for the specific coenzymes F420 and 7-methylpterin, respectively. In contrast to methylpterin, the fluorescence of F420 was bleached selectively, with a time constant of about 160 s, at an irradiation power density of 5 mW mm-2. Similar time constants were found for samples of sewage sludge containing methanogenic bacteria. Active and inactive bacterial cells could be differentiated by following the course of photobleaching.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0003-2670
e-ISSN
1873-4324
Journal
Analytica Chimica Acta
Quellenangaben
Volume: 163,
Issue: C,
Pages: 249-255
Publisher
Elsevier
Reviewing status
Peer reviewed
Institute(s)
Abteilung Biophysikalische Strahlenforschung