PuSH - Publication Server of Helmholtz Zentrum München: Time-resolved fluorescence microscopy for measuring specific coenzymes in methanogenic bacteria.

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Schneckenburger, H. ; Reuter, B.W. ; Schoberth, S.M.*

Time-resolved fluorescence microscopy for measuring specific coenzymes in methanogenic bacteria.

Anal. Chim. Acta 163, 249-255 (1984)

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Measurements of time-resolved photobleaching and nanosecond fluorescence decay from microscopic samples of methanogenic bacteria are reported. From cultures of Methanobacterium thermoautotrophicum and Methanosarcina barkeri, decay times of 1 ns and 3 ns were obtained for the specific coenzymes F420 and 7-methylpterin, respectively. In contrast to methylpterin, the fluorescence of F420 was bleached selectively, with a time constant of about 160 s, at an irradiation power density of 5 mW mm-2. Similar time constants were found for samples of sewage sludge containing methanogenic bacteria. Active and inactive bacterial cells could be differentiated by following the course of photobleaching.

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