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Open Access Green as soon as Postprint is submitted to ZB.
Separation of pteridines from blood cells and plasma by reverse-phase high-performance liquid chromatography.
Anal. Biochem. 133, 68-78 (1983)
In order to determine free and conjugated pteridines, the pteridine-releasing procedure for biological materials (e.g., plasma, blood cells, bone marrow) was optimized and the parameters which influence pteridine release are discussed. Using reverse-phase high-performance liquid chromatography (rp-HPLC) on 5-μm ODS (C18; mobile phase, K2HPO4 buffer, pH 7.0-7.8), up to 10 pteridines (pterin-6-carboxylic acid, xanthopterin, neopterin, monapterin, isoxanthopterin, lumazine, biopterin, 6-hydroxymethylpterin, pterin) could be separated in the extracts of blood samples and determined fluorometrically (femtomolar range). In addition, three sepiapterins (sepiapterin, deoxysepiapterin, 3'-hydroxysepiapterin) could be separated with aqueous methanol as eluent (uv and fluorometric detection, respectively). The methods described are suited to compile pteridine patterns for plasma and individual cell fractions (erythrocytes, lymphocytes, buffy coat) from blood. Moreover, it was shown that the pteridine concentrations in dog blood (beagles) were in some cases substantially higher than in human blood, and that neopterin is lacking in the blood cell fractions of beagle dogs or occurs only in very low concentrations.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0003-2697
e-ISSN
1096-0309
Journal
Analytical Biochemistry
Quellenangaben
Volume: 133,
Issue: 1,
Pages: 68-78
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Hämatologie