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Open Access Green as soon as Postprint is submitted to ZB.
Discrimination in immunofluorescence between labeled and unlabeled cells by quantitative microfluorometry and computer analysis.
J. Immunol. Methods 40, 155-163 (1981)
An automated procedure for discrimination in immunofluorescence between antibody-labeled and unlabeled cells has been developed on the basis of microfluorimetric determination of intensity distributions. After smoothing the raw data for irregularities caused by the scoring statistics optimum fit of the negative distribution to the corresponding positive one was achieved. The procedure was tested in a model system by mixing various known proportions of immunofluorescence-negative and positive plastic beads. In addition, variable mixtures of T-negative CLL cells and normal mononuclear peripheral blood cells were labeled with FITC-conjugated anti-T-antiserum. The expected percentage of T-positive peripheral blood cells agreed satisfactorily with the data measured and computed. Finally, the measured percentage of Ig-positive mononuclear cells from normal peripheral blood was in agreement with the values obtained by other techniques.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0022-1759
e-ISSN
1872-7905
Journal
Journal of Immunological Methods
Quellenangaben
Volume: 40,
Issue: 2,
Pages: 155-163
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Hämatologie