PuSH - Publication Server of Helmholtz Zentrum München: Mast cell growth-enhancing activity (MEA) stimulates interleukin 6 production in a mouse bone marrow-derived mast cell line and a malignant subline.

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Hültner, L. ; Moeller, J.

Mast cell growth-enhancing activity (MEA) stimulates interleukin 6 production in a mouse bone marrow-derived mast cell line and a malignant subline.

Exp. Hematol. 18, 873-877 (1990)

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Open Access Green as soon as Postprint is submitted to ZB.

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A novel mast cell growth-enhancing activity (MEA/P40/interleukin 9 [IL-9]) purified from the conditioned medium of a murine interleukin 2 (IL-2)-dependent Mlsa-specific T-cell line (MLS4.2) was tested for its capacity to induce interleukin 6 (IL-6) production in a mouse bone marrow-derived factor-dependent mast cell line (L138.8A). This interleukin 3 (IL-3)/interleukin 4 (IL-4)/MEA-responsive cell line was demonstrated recently to express IL-6 mRNA and to secrete IL-6 when cultured with IL-3/IL-4. Now we were able to show that conditioned medium from L138.8A mast cells stimulated with MEA alone contained growth factor activity for the IL-6-dependent mouse hybridoma cell line 7TD1 that was completely blocked by the monoclonal anti-IL-6 antibody 6B4. A dose-response study including IL-3, IL-4, and MEA tested either alone or in different combinations revealed that among these growth factors MEA was the most potent inducer of IL-6 in L138.8A cells. Moreover, IL-4 but not IL-3 had a strong synergistic effect on MEA-induced IL-6 production. The autonomous malignant mast cell subline L138Cauto also showed enhanced IL-6 production when stimulated with MEA. Our findings indicate that MEA (IL-9) not only provides a proliferation signal, but also leads to a marked functional activation of responsive mast cells.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Il-3 ; Il-4 ; Il-6 ; Il-9 ; Mast Cell Growth-enhancing Activity ; Mouse Bone Marrow-derived Mast Cells ; P40

ISSN (print) / ISBN 0301-472X

e-ISSN 0301-472X

Journal Experimental Hematology

Quellenangaben Volume: 18, Issue: 8, Pages: 873-877

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institut für Experimentelle Hämatologie