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Effect of sulfadiazine on abundance and diversity of denitrifying bacteria by determining nirK and nirS genes in two arable soils.
Microb. Ecol. 60, 703-707 (2010)
Sulfadiazine (SDZ) is an antibiotic frequently used in agricultural husbandry. Via manuring of excrements of medicated animals, the drug reaches the soil and might impair important biochemical transformation processes performed by microbes, e.g., the nitrogen turnover. We studied the effect of pig manure and SDZ-spiked pig manure on denitrifying bacteria by quantifying nirK and nirS nitrite reductase genes in two arable soils. Addition of manure entailed mainly an increase of nirK-harboring denitrifiers in both soils, whereas in the SDZ-amended treatments, primarily the nirS denitrifiers increased in abundance after the bioavailable SDZ had declined. However, the community composition of nirS nitrite reducers investigated by denaturing gradient gel electrophoresis did not change despite the observed alterations in abundance.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
PIG-MANURE; AGRICULTURAL SOILS; COMMUNITY; DENITRIFICATION; NITRIFICATION; INHIBITION; DYNAMICS; NITRATE; SAMPLES
ISSN (print) / ISBN
0095-3628
e-ISSN
1432-184X
Journal
Microbial Ecology
Quellenangaben
Volume: 60,
Issue: 4,
Pages: 703-707
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Soil Ecology (IBOE)