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Open Access Green as soon as Postprint is submitted to ZB.
S1 nuclease-sensitive sites in yeast DNA: An assay for radiation-induced base damage.
Int. J. Radiat. Biol. 45, 497-504 (1984)
DNA has been isolated from γirradiated yeast cells and then treated with the enzyme S1 nuclease. This enzyme cleaves DNA specifically at sites where localized denaturation has occurred and can therefore be used as a lesion probe to identify regions in the DNA where base-pairing has been disrupted. By analysing the number of single strand breaks, double strand breaks and alkali-labile sites in the DNA before and after treatment with S1 nuclease, it has been possible to calculate the number of S1 nuclease-sensitive sites induced as a result of exposure to ionizing radiation. These sites were found to occur with a frequency about twice that of the double strand break and are thought to result from a primary ionization event in the DNA.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Base Damage ; Dna ; Nuclease-sensitive Sites ; Yeast
ISSN (print) / ISBN
0955-3002
e-ISSN
1362-3095
Quellenangaben
Volume: 45,
Issue: 5,
Pages: 497-504
Publisher
Informa Healthcare
Reviewing status
Peer reviewed
Institute(s)
Abteilung für Strahlenbiologie und Biophysik