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Conrad, R.S. ; Schlegel, H.G.

Different degradation pathways for glucose and fructose in Rhodopseudomonas capsulata.

Arch. Microbiol. 112, 39-48 (1977)
Publ. Version/Full Text DOI PMC
In Rhodopseudomonas capsulata the enzymes of the Entner-Doudoroff pathway and the Embden-Meyerhof pathway have been examined. Fructose-grown cells contained inducible activities of phosphoenolpyruvate-fructosephospho-transferase and 1-phosphofructokinase and only low levels of fructokinase and 6-phosphofructokinase. Although fructose-grown cells contained, in addition, all the enzymes of the Entner-Doudoroff pathway together with fructose-1,6-diphosphatase and phosphoglucose isomerase, the Entner-Doudoroff pathway was not operative in fructose catabolism and served only the degradation of glucose. The functional separation of glucose and fructose catabolism via the Entner-Doudoroff and a modified Embden-Meyerhof pathway, respectively, was confirmed by different approaches: 1. Radiorespirometric experiments with glucose and fructose labelled in positions 1, 2, 3+4 and 6 have been carried out. The pattern of 14CO2-evolution from position-labelled glucose was characteristic for the Entner-Doudoroff pathway, that from position-labelled fructose for the Embden-Meyerhof pathway. 2. In the presence of arsenite up to 50% of glucose-and fructose-carbon was excreted as pyruvate. Using 1-14C-glucose, 86% of the pyruvate was labelled in the carboxyl group, whereas using 1-14C-fructose only 19% of the pyruvate was labelled in the carboxyl group. 3. A glucose-6-phosphate dehydrogenase-deficient mutant was isolated which lacked a functional Entner-Doudoroff pathway but which was unaltered in its ability to grow on fructose.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Embden-meyerhof Pathway ; Entner-doudoroff Pathway ; Hexose Catabolism ; Mutant Isolation ; Pep-fructose-phosphotransferase ; Radiometric Techniques ; Rhodopseudomonas Capsulata
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Journal Archives of Microbiology
Quellenangaben Volume: 112, Issue: 1, Pages: 39-48 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institut für Mikrobiologie