PuSH - Publication Server of Helmholtz Zentrum München: Identification and quantitative determination of the flavin component of soluble hydrogenase from Alcaligenes eutrophus.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Schneider, K.A. ; Schlegel, H.G.

Identification and quantitative determination of the flavin component of soluble hydrogenase from Alcaligenes eutrophus.

Biochem. Biophys. Res. Commun. 84, 564-571 (1978)

DOI

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

The flavin component of soluble hydrogenase (hydrogen: NAD+ oxidoreductase, EC 1.12.1.2) from Alcaligenes eutrophus was identified as FMN by thin layer chromatography in two solvent systems and by binding studies with apoflavodoxin from Megasphaera elsdenii. The flavin of hydrogenase reacted rapidly with apoflavodoxin with almost complete quenching of the fluorescence at 525 nm. Quantitative determination of FMN was performed by fluorimetric titration with a standardized solution of apoflavodoxin. From the determined FMN content of different enzyme preparations and from the percentage of stimulation of hydrogenase activity by exogenous FMN it is concluded that hydrogenase contains 2 FMN per molecule.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0006-291X

e-ISSN 1090-2104

Journal Biochemical and Biophysical Research Communications

Quellenangaben Volume: 84, Issue: 3, Pages: 564-571

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institut für Mikrobiologie