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Open Access Green as soon as Postprint is submitted to ZB.
Identification and quantitative determination of the flavin component of soluble hydrogenase from Alcaligenes eutrophus.
Biochem. Biophys. Res. Commun. 84, 564-571 (1978)
The flavin component of soluble hydrogenase (hydrogen: NAD+ oxidoreductase, EC 1.12.1.2) from Alcaligenes eutrophus was identified as FMN by thin layer chromatography in two solvent systems and by binding studies with apoflavodoxin from Megasphaera elsdenii. The flavin of hydrogenase reacted rapidly with apoflavodoxin with almost complete quenching of the fluorescence at 525 nm. Quantitative determination of FMN was performed by fluorimetric titration with a standardized solution of apoflavodoxin. From the determined FMN content of different enzyme preparations and from the percentage of stimulation of hydrogenase activity by exogenous FMN it is concluded that hydrogenase contains 2 FMN per molecule.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0006-291X
e-ISSN
1090-2104
Quellenangaben
Volume: 84,
Issue: 3,
Pages: 564-571
Publisher
Elsevier
Reviewing status
Peer reviewed
Institute(s)
Institut für Mikrobiologie