Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Effective avidin coating of erythrocytes by disulfide bond formation : Production of a stable rosetting agent for multiple applications.
J. Immunol. Methods 91, 123-128 (1986)
A method is described for covalently coupling avidin to the membrane of glutaraldehyde-treated erythrocytes. The method is based on the use of the heterobifunctional coupling reagent N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) and appears to be more effective than other protein coupling techniques. The resulting avidin-coated red cells are very stable and can be stored for more than 1 year without aggregation or loss of biotin-binding activity. They can be used in various rosetting assays and were tested for T cell depletion or negative cell selection with five biotinylated monoclonal antibodies.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Avidin-biotin; Cell separation; Coating erythrocyte; Rosetting
ISSN (print) / ISBN
0022-1759
e-ISSN
1872-7905
Journal
Journal of Immunological Methods
Quellenangaben
Volume: 91,
Issue: 1,
Pages: 123-128
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Hämatologie