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Schneckenburger, H. ; Reuter, B.W. ; Schoberth, S.M.*

Time-resolved microfluorescence for measuring coenzyme F420 in Methanobacterium thermoautotrophicum.

FEMS Microbiol. Lett. 22, 205-208 (1984)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The time course of photobleaching and the nanosecond fluorescence decay have been measured from microscopic samples of methanogenic bacteria, to our knowledge the first application of these methods in this field. Decay times of about 1 ns and 3 ns were obtained for the specific coenzymes F420 and 7-methylpterin, respectively. In contrast to methylpterin and other fluorescent compounds the intensity of F420 fluorescence was reduced selectively due to photobleaching. This effect, as well as the different decay time constants could be used to discriminate F420 from other fluorescent components. In addition, active and inactive bacterial cells could be differentiated following the course of photobleaching.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords 7-methylpterin ; Autofluorescence ; Fluorescence Decay ; Methanogenic Bacteria ; Photobleaching
ISSN (print) / ISBN 0378-1097
e-ISSN 1574-6968
Journal FEMS Microbiology Letters
Quellenangaben Volume: 22, Issue: 3, Pages: 205-208 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed