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Munker, R. ; Stünkel, K.G.E. ; Thiel, E.V. ; Thierfelder, S.S.

Analysis with monoclonal antibodies of human lymphoid cells forming rosettes with rabbit red blood cells.

Clin. Exp. Immunol. 51, 479-486 (1983)

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Open Access Green as soon as Postprint is submitted to ZB.

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Rabbit red blood cells have previously been shown to rosette with a subpopulation of thymocytes and with mitogen activated peripheral lymphocytes but not with unstimulated lymphocytes. Using monoclonal antibodies and double marker assays we studied the phenotype of these cells. In thymus, over 90% of rosetting cells express antigens of immature thymocytes (HTA1, OKT6). A proportion of the rosetting cells shows in addition antigens of mature thymocytes (OKT3, UCHT1). These cells probably correspond to a stage of intrathymic maturation between common and mature thymocytes. Virtually all rosetting cells are T cells and express an antigen related to T cell activation (TAC) when lymphocytes are activated by mitogens like PHA or Con A. Few rosetting cells are Ia positive. Two other antigens (OKT9, OKT10) known to be associated with proliferating and immature cells, are found in variable proportions on rosetting cells. After stimulation with allogeneic lymphocytes, fewer rosettes are detected than after stimulation by mitogens. Cells activated by a soluble antigen (PPD) and forming rosettes with rabbit red blood cells have a helper phenotype (Leu3a positive). Screening of leukaemia cell samples revealed that only cells from patients with T-ALL form rosettes with rabbit red blood cells. Rosette formation is almost totally inhibited by a polyclonal anti-thymocyte serum and two monoclonal antibodies (OKT11A, Lyt3) which have been shown to block rosettes with sheep erythrocytes.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0009-9104

e-ISSN 1365-2249

Journal Clinical & Experimental Immunology

Quellenangaben Volume: 51, Issue: 3, Pages: 479-486

Publisher Wiley

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institut für Hämatologie