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Localization of ribosomal cistrons in metaphase chromosomes of Vicia faba (L.).

Exp. Cell Res. 90, 463-465 (1975)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Tritiated ribosomal RNA (rRNA) was prepared from the roots of Vicia faba after incubation in 3H uridine. Separation of the nucleic acids MAK chromatography yielded fractions of specific activity of 4-5 x 105 dpm/μg. 4 + 5S, 18S and 25S RNA fractions were used for cytological hybridization on squash preparations of Vicia faba root tip meristems. Autoradiographs of the 18S and 25S RNA preparations exhibited a clear labelling in the secondary constriction of the satellite (SAT) chromosomes after exposition times of 28 weeks.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 1090-2422
e-ISSN 0014-4827
Quellenangaben Volume: 90, Issue: 2, Pages: 463-465 Article Number: , Supplement: ,
Publisher Academic Press
Publishing Place Orlando, Fla.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institut für Strahlenbotanik