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Open Access Green as soon as Postprint is submitted to ZB.
Localization of ribosomal cistrons in metaphase chromosomes of Vicia faba (L.).
Exp. Cell Res. 90, 463-465 (1975)
Tritiated ribosomal RNA (rRNA) was prepared from the roots of Vicia faba after incubation in 3H uridine. Separation of the nucleic acids MAK chromatography yielded fractions of specific activity of 4-5 x 105 dpm/μg. 4 + 5S, 18S and 25S RNA fractions were used for cytological hybridization on squash preparations of Vicia faba root tip meristems. Autoradiographs of the 18S and 25S RNA preparations exhibited a clear labelling in the secondary constriction of the satellite (SAT) chromosomes after exposition times of 28 weeks.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1090-2422
e-ISSN
0014-4827
Journal
Experimental Cell Research
Quellenangaben
Volume: 90,
Issue: 2,
Pages: 463-465
Publisher
Academic Press
Publishing Place
Orlando, Fla.
Reviewing status
Peer reviewed
Institute(s)
Institut für Strahlenbotanik