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Schneider, K.A. ; Schlegel, H.G.

Localization and stability of hydrogenases from aerobic hydrogen bacteria.

Arch. Microbiol. 112, 229-238 (1977)
Publ. Version/Full Text DOI
Alcaligenes eutrophus strains H 16, B 19, G 27 and N9A contained two different hydrogenases. One enzyme catalyzed the reduction of NAD by hydrogen and was strictly localized in the soluble cell fraction, while the second enzyme was found to be particulate and unable to react with NAD. All other tested strains, Alcaligenes paradoxus SA 29, Pseudomonas facilis, P. palleronii RH 2, Pseudomonas sp. strain GA 3, Paracoccus denitrificans, Aquaspirillum autotrophicum SA 32, and Corynebacterium autotrophicum 14g and 7C contained only a single enzyme exclusively bound to membranes. This was established using fractional centrifugation, indicator enzyme systems, gentle methods of cell disintegration and discontinuous sucrose density gradient centrifugation. In cell-free extracts obtained by rough disruption (sonication) of cells, hydrogenase was associated to particles of different size and sedimentation velocity. A partial solubilization of hydrogenase caused by sonication was observed with P. facilis. Without exception, the particulate hydrogenases were found (1) to be unable to reduce pyridine nucleotides, and (2) to reduce methylene blue at an extremely high activity. The eminent reaction rate of 34 μmoles H2 oxidized per min and mg protein has been determined in particle suspensions of Pseudomonas sp. strain GA 3. All hydrogenases were stable during storage under hydrogen atmosphere, except the soluble enzyme from A. eutrophus H 16 which was shown to be more stable under aerobic conditions.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Hydrogen Bacteria ; Hydrogenase ; Localization ; Membrane Bound Enzymes ; Nad Reduction ; Stability
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Journal Archives of Microbiology
Quellenangaben Volume: 112, Issue: 3, Pages: 229-238 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institut für Mikrobiologie