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Evidence for the in vivo regulation of glucose 6-phosphate dehydrogenase activity in Hydrogenomonas eutropha H 16 from measurements of the intracellular concentrations of metabolic intermediates.
Arch. Microbiol. 97, 273-281 (1974)
The inhibition of fructose utilization by whole cells of Hydrogenomonas eutropha H 16, following the addition of hydrogen to the gas phase, has been explained as an inhibition of glucose 6-phosphate dehydrogenase (Blackkolb and Schlegel, 1968a, b). The intracellular concentrations of glucose 6-phosphate, 6-phosphogluconate, three inhibitors of the enzyme (NADH, ATP and phospho enolpyruvate) and some related metabolites were measured in cells incubated in the presence and absence of hydrogen. Inhibition of glucose 6-phosphate dehydrogenase was confirmed by an increase in the glucose 6-phosphate pool and a decrease in the 6-phosphogluconate concentration. The regulatory control is apparently due to a threefold increase in the NADH concentration while the concentrations of the other two inhibitors fell slightly. When the measured intracellular concentrations of intermediates were used in the in vitro assay of glucose 6-phosphate dehydrogenase activity, an almost total inhibition of the dehydrogenase was observed, therefore further regulatory factors must be considered.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Enzyme Regulation ; Glucose 6-phosphate Dehydrogenase ; Intracellular Concentrations ; Pool Size (pep, Atp, Adp, Amp, Nad, Nadh, G-6-p, 6-pg, F-6-p)
ISSN (print) / ISBN
0003-9276
e-ISSN
1432-072X
Journal
Archives of Microbiology
Quellenangaben
Volume: 97,
Issue: 1,
Pages: 273-281
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Mikrobiologie