Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Simultaneous enrichment of HL-60 cells in G1 and separation from differentiating cells by centrifugal elutriation for studies on differentiation induction.
Exp. Cell Res. 169, 524-530 (1987)
Cultures of the promyelocytic leukemia cell line HL-60 usually contain considerable numbers of spontaneously differentiating cells and are asynchronous in terms of cell-cycle phases. Counterflow centrifugal elutriation studies have been conducted to obtain a homogeneous cell population with regard to cell-cycle phases and stage of differentiation. Despite their small volume and probably because of their high buoyant density, differentiated cells are elutriated predominantly at higher flow rates. Accordingly, G1 cells elutriated at low flow rates are substantially free from spontaneously differentiating cells. By optimizing the technique, a population with approx. 90% G1 cells and less than 1% spontaneously differentiating cells was obtained. The yield in the fractions chosen was 5.1% of all cells recovered from elutriation. In culture, a cell population of this purity maintains a synchronous cell cycle for more than 2 days. This allows an exact determination of the time after induction when first signs of differentiation occur. The presence of 1 μM retinoic acid (RA) causes the first significant increase of NBT-positive cells between the 24th and 27th h of culture.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1090-2422
e-ISSN
0014-4827
Journal
Experimental Cell Research
Quellenangaben
Volume: 169,
Issue: 2,
Pages: 524-530
Publisher
Academic Press
Publishing Place
Orlando, Fla.
Reviewing status
Peer reviewed
Institute(s)
Institut für Hämatologie