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Open Access Green as soon as Postprint is submitted to ZB.
Elimination of mycoplasmas from cell cultures and establishment of mycoplasma-free cell lines.
Exp. Cell Res. 152, 565-570 (1984)
Several antibiotics were examined for their potential to eliminate mycoplasmas from contaminated cell cultures. Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma hyorhinis and Mycoplasma orale were effectively eliminated from experimentally contaminated mouse fibroblasts and mink epithelial cells by the use of the antibiotics minocycline and tiamutin. An elimination procedure was established, which involved the consecutive treatment of the cultures over a period of 3 weeks, followed by cell cloning. This procedure was effective when applied to cell lines which had been contaminated with unidentified and partially non-cultivable strains of mycoplasmas.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1090-2422
e-ISSN
0014-4827
Journal
Experimental Cell Research
Quellenangaben
Volume: 152,
Issue: 2,
Pages: 565-570
Publisher
Academic Press
Publishing Place
Orlando, Fla.
Reviewing status
Peer reviewed
Institute(s)
Institute of Pathology (PATH)