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Open Access Green as soon as Postprint is submitted to ZB.
Quantitative determination of retrovirus-specific immune complexes.
J. Immunol. Methods 134, 113-119 (1990)
ELISA methodology was adapted to measure antigen-specific immune complexes (ASIC) containing retroviral antigens. Using ICs artificially generated from MuLV gp70 and monoclonal antibodies against gp70 we showed that ICs can be quantified by measuring the levels of antibody and antigen and comparing these to the total content of ICs. Furthermore, this method permits determination of the composition of ICs containing an excess of antigen or antibody (i.e., small or large complexes). The levels of the ASIC were correlated with the leves of ICs containing undefined antigen components as determined by the C1q ELISA. Using this method it was possible to determine levels of MuLV gp70 specific ICs in various mouse sera.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Antigen Specific Immune Complexes ; Detection And Quantification ; Elisa
ISSN (print) / ISBN
0022-1759
e-ISSN
1872-7905
Journal
Journal of Immunological Methods
Quellenangaben
Volume: 134,
Issue: 1,
Pages: 113-119
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed