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Open Access Green as soon as Postprint is submitted to ZB.
Improved detection of terminal transferase (TdT): The use of detergents on glutaraldehyde-fixed non-dehydrated cells prevents denaturation and diffusion artifacts.
Leuk. Res. 10, 1041-1049 (1986)
TdT as an intranuclear enzyme mainly of immature lymphoid cells is commonly determined immunologically using air-dried cell smears fixed with methanol. Both cell dehydration and alcohol fixation were found here to denature TdT and surface antigens. This could be prevented by using non-dehydrated cells bound electrostatically to poly-L-lysine-coated slides, fixed minimally with glutaraldehyde and rendered permeable to antibodies by the non-ionic detergent Brij 56. Crosslinking glutaraldehyde in addition prevented diffusion of TdT to extranuclear sites. By avoiding artifacts of denaturation and diffusion, a higher sensitivity in the detection of TdT was achieved despite considerably lower quantities of antibody.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Detergents; glutaraldehyde; immunocytochemistry; methanol; polylysine; sensitivity; terminal transferase
ISSN (print) / ISBN
0145-2126
e-ISSN
1873-5835
Journal
Leukemia Research
Quellenangaben
Volume: 10,
Issue: 8,
Pages: 1041-1049
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed