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Open Access Green as soon as Postprint is submitted to ZB.
Kinetics of myeloid progenitor cells in human micro long-term bone marrow cultures.
Exp. Hematol. 16, 145-149 (1988)
Hemopoiesis was analyzed in a miniaturized long-term culture of human bone marrow cells by quantifying the production of granulocyte-macrophage progenitor cells. As in the conventional long-term culture system, hemopoiesis was dependent on the presence of a marrow-derived adherent layer. Adipocytes proved to be essential for long-term proliferation. Optimal growth conditions were maintained by incubation in McCoy's medium supplemented with hydrocortisone, fetal calf serum, and horse serum. When calculated back to the volume of conventional cultures, the numbers and kinetics of nucleated cells and granulocyte-macrophage colony-forming cells were comparable in both culture systems. The microsystem is therefore suitable for performing multiple analyses on small samples of cells.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0301-472X
e-ISSN
0301-472X
Journal
Experimental Hematology
Quellenangaben
Volume: 16,
Issue: 2,
Pages: 145-149
Publisher
Elsevier
Reviewing status
Peer reviewed
Institute(s)
Institut für Hämatologie