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Jäger, G. ; Lau, B. ; Pachmann, K. ; Dörmér, P.G.

Acute lymphoblastic leukaemia cells in the diffusion chamber: Change of surface phenotype during the culture period.

Br. J. Haematol. 52, 319-325 (1982)

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Open Access Green as soon as Postprint is submitted to ZB.

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Peripheral blast cells from patients with acute lymphoblastic leukaemia were cultured in the diffusion chamber system. On different harvesting days the cALL antigen, surface immunoglobulin and T-cell antigen were investigated by direct immunofluorescence. Each cell type investigated showed a different response throughout culture, the commonest change observed being development into the B-cell axis. Our data demonstrate that the differentiation stop of a leukaemic cell can be overcome by using the diffusion chamber system. The data therefore give some insight into the stage of developmental arrest in acute lymphoblastic leukaemia.

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