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Microbial metabolism of haloaromatics: Isolation and properties of a chlorobenzene-degrading bacterium.
Appl. Environ. Microbiol. 47, 395-402 (1984)
A chlorobenzene-degrading bacterium was isolated by continuous enrichment from a mixture of soil and sewage samples. This organism, strain WR1306, was grown in a chemostat on a mineral medium with chlorobenzene being supplied through the vapor phase with a critical D(c) value at a dilution rate of 0.55 h-1. Maximum growth rates in batch culture were accomplished at substrate concentrations of ≤0.5 mM in the culture medium. During growth on chlorobenzene, stoichiometric amounts of chloride were released. Respiration data and enzyme activities in cell extracts as well as the isolation of 3-chlorocatechol from the culture fluid are consistent with the degradation of chlorobenzene via 3-chloro-cis-1,2-dihydroxycyclohexa-3,5-diene, 3-chlorocatechol, 2-chloro-cis,cis-muconate, trans-4-carboxymethylenebut-2-en-4-olide, maleylacetate, and 3-oxoadipate.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0099-2240
e-ISSN
1098-5336
Quellenangaben
Volume: 47,
Issue: 2,
Pages: 395-402
Publisher
American Society for Microbiology (ASM)
Reviewing status
Peer reviewed
Institute(s)
Departments & Institutes