PuSH - Publication Server of Helmholtz Zentrum München: Macrophage colony-stimulating activity is produced by three different EBV-transformed lymphoblastoid cell lines.

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Reisbach, G. ; Hültner, L. ; Kranz, B.R. ; Scholz, S.G. ; Wolf, H.J. ; Dörmér, P.G.

Macrophage colony-stimulating activity is produced by three different EBV-transformed lymphoblastoid cell lines.

Cell. Immunol. 109, 246-254 (1987)

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Open Access Green as soon as Postprint is submitted to ZB.

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The lymphoblastoid cell lines BLY 9.84, Gl, and Pl constitutively release a colony-stimulating activity (CSA) which specifically stimulates murine macrophage progenitor cells in vitro. The biochemical characterization of BLY 9.84-derived CSA exhibits a molecular size of apparently 150-200 kDa even under dissociating conditions with 6 M guanidine hydrochloride or inhibition of glycosylation. Production of this CSA is inhibited by cycloheximide and its activity is destroyed by reduction with dithiothreitol. Replating experiments give evidence for a functional similarity with CSF-1 (macrophage colony-stimulating factor).

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0008-8749

e-ISSN 0008-8749

Journal Cellular Immunology

Quellenangaben Volume: 109, Issue: 1, Pages: 246-254

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed