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Graw, J. ; Gopinath, P.M. ; Bors, W. ; Michel, C. ; Summer, H.K.H.

Biochemical analysis of young rats homozygous for the cataract mutation cat.

Exp. Eye Res. 48, 1-9 (1989)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Cataractogenesis was studied in young rats homozygous for the radiation-induced recessive cataract mutation cat. Homozygous cat/cat rats have reduced body weight (about two-thirds of the wild type) when 3 weeks old. The litter size is also diminished to about two-thirds of the wild type. For lens-specific parameters, as compared with homozygous wild type, the wet weight of the cataractous lenses is reduced, although the concentration of water-soluble lens proteins per wet weight is the same. No major alterations could be detected in the pattern of the water-soluble lens proteins separated by isoelectric focusing or gel electrophoresis run with or without mercaptoethanol. Additionally, no statistically significant alterations could be detected in the biochemical parameters of the lens used as indicators for osmotic stress (water content of the lens and the Na+-K+-dependent ATPase), for the energy state (ATP) and for the redox state (oxidized glutathione and superoxide dismutase). In contrast, the activity of transglutaminase is significantly enhanced in lenses as well as in the liver of young cat-rats, which might be understood as a biochemical marker for alterations in the developmental program. Cataractogenesis in the cat-rat is, therefore, suggested to be part of a syndrome including dwarfism and reduced litter size.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Atp ; Cataract ; Crystallin ; Glutathione ; Rat ; Superoxide Dismutase ; Transglutaminase
ISSN (print) / ISBN 0014-4835
e-ISSN 1096-0007
Journal Experimental Eye Research
Quellenangaben Volume: 48, Issue: 1, Pages: 1-9 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)