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Pohlit, W. ; Heyder, I.R.

The shape of dose-survival curves for mammalian cells and repair of potentially lethal damage analyzed by hypertonic treatment.

Radiat. Res. 87, 613-634 (1981)

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During the usual procedure of testing cell survival by colony-forming ability, repair of potentially lethal damage (PLD) takes place. By incubating the cells in hypertonic suspension a certain part of this repair can be inhibited, leading to an exponential dose-survival curve as expected from the Poisson distribution of lethal events in the cells. If such a hypertonic treatment is performed after increasing intervals following irradiation with X rays, curves with increasing shoulder length are obtained. Quantitative analysis of the kinetics of this repair shows that PLD is repaired for about 1 hr after irradiation by a saturated repair system which eliminates about one lesion per 15 min per cell independent of the applied absorbed dose. PLD not eliminated by this 'fast' system is repaired by an unsaturated system with a time constant of several hours. Repair of PLD after X irradiation proceeds quantitatively in this way in plateau-phase cells suspended in a conditioned medium, which seems optimal for such repair. If these cells are suspended after irradiation in normal nutrient medium a certain fraction of the PLD is transformed into irreparable damage. The final survival after repair in nutrient medium is then identical with that obtained by the usual measurement of colony-forming ability on nutrient agar. This indicates that the shoulder in dose-survival curves for plateau-phase cells is partly due to repair of PLD and partly due to manifestation of this damage during repair time.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0033-7587

e-ISSN 1938-5404

Journal Radiation Research

Quellenangaben Volume: 87, Issue: 3, Pages: 613-634

Publisher Radiation Research Society

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Abteilung Biophysikalische Strahlenforschung