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Da Ines, O. ; Graf, W. ; Franck, K.I. ; Albert, A. ; Winkler, J.B. ; Scherb, H. ; Stichler, W. ; Schäffner, A.

Kinetic analyses of plant water relocation using deuterium as tracer - reduced water flux of Arabidopsis pip2 aquaporin knockout mutants.

Plant Biol. 12, (Suppl.1), 129-139 (2010)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Due to reduced evaporation and diffusion of water molecules containing heavier isotopes, leaf water possesses an elevated (18)O or (2)H steady-state content. This enrichment has been exploited in plant physiology and ecology to assess transpiration and leaf water relations. In contrast to these studies, in this work the (2)H content of the medium of hydroponically grown Arabidopsis thaliana was artificially raised, and the kinetics of (2)H increase in the aerial parts recorded during a short phase of 6-8 h, until a new equilibrium at a higher level was reached. A basic version of the enrichment models was modified to establish an equation that could be fitted to measured leaf (2)H content during uptake kinetics. The fitting parameters allowed estimation of the relative water flux q(leaf) into the Arabidopsis rosette. This approach is quasi-non-invasive, since plants are not manipulated during the uptake process, and therefore, offers a new tool for integrated analysis of plant water relations. The deuterium tracer method was employed to assess water relocation in Arabidopsis pip2;1 and pip2;2 aquaporin knockout plants. In both cases, q(leaf) was significantly reduced by about 20%. The organ and cellular expression patterns of both genes imply that changes in root hydraulic conductivity, as previously demonstrated for pip2;2 mutants, and leaf water uptake and distribution contributed in an integrated fashion to this reduced flux in intact plants.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Arabidopsis; D2O; Kinetics of enrichment; Non-invasive analysis; Stable isotope; Water flux
ISSN (print) / ISBN 1435-8603
e-ISSN 1438-8677
Journal Plant Biology
Quellenangaben Volume: 12, Issue: SUPPL. 1, Pages: 129-139, Article Number: , Supplement: (Suppl.1)
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)
Research Unit Environmental Simulation (BIOP-EUS)
Institute of Biomathematics and Biometry (IBB)
Institute of Groundwater Ecology (IGOE)