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Comparison of methods to test chemicals for side effects on soil microorganisms.

Ecotoxicol. Environ. Saf. 12, 53-69 (1986)
DOI PMC
The effects of the test chemicals pentachlorophenol (PCP) and HgCl2 on the bioactivity of microorganisms in three different soils were studied in low and high concentrations (2 and 20 ppm). Bioactivity was measured in long-term experiments (18 weeks) by a threefold application of chemicals to soils of varying moisture content. The selected tests were measurements of ATP, of heat output under aerobic and anaerobic conditions and after amendment with glucose, of soil respiration after the addition of glucose, and of Fe(III) reduction. The suitability of each test depends on soil microorganisms, on environmental conditions, and on soil properties. The effects caused by the chemicals were as follows. For the low concentration, stimulation or inhibition were mostly repaired within the observation period. The high concentration mostly induced inhibitions which increased or decreased as a consequence of the repeated application of the chemicals. The effects of chemicals were strongly modified by the soil types: in a soil with high organic matter content, differences to the control were lower than in soils with lower organic matter content. These experiments also indicate that measurement of only one physiological parameter is not sufficient to characterize chemicals ecotoxicologically.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0147-6513
e-ISSN 0147-6513
Quellenangaben Volume: 12, Issue: 1, Pages: 53-69 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)