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Kohfeldt, E. ; Bertram, H. ; Hagen, U.

Action of gamma endonuclease on clustered lesions in irradiated DNA.

Radiat. Environ. Biophys. 27, 123-132 (1988)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Irradiation of DNA in situ i.e. in phage particles or in the cell leads to alterations of single DNA nucleotides as well as to clustered lesions such as double strand breaks or unpaired DNA regions the latter being sensitive to digestion by S 1 nuclease. A contribution will be made to the configuration of such S 1-nuclease-sensitive sites (S 1 sites). DNA from irradiated lambda phage containing S 1 sites was treated with gamma endonuclease from M. luteus which is known to split the nucleotide strand at the position of oxidized pyrimidine base. It was found that the gamma endonuclease induces double-strand breaks at some of the S 1 sites indicating double base damage within this site. However, half of the S 1 sites are not converted into a double-strand break by the gamma endonuclease, indicating base damage only on one strand within the unpaired region.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0301-634X
e-ISSN 1432-2099
Journal Radiation and Environmental Biophysics
Quellenangaben Volume: 27, Issue: 2, Pages: 123-132 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)