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Open Access Green as soon as Postprint is submitted to ZB.
The Cd-Chelex assay: A new sensitive method to determine metallothionein containing zinc and cadmium.
Arch. Toxicol. 64, 177-180 (1990)
A rapid and sensitive one-vial procedure to determine metallothionein (MT) containing zinc (Zn) and cadmium (Cd) is described. New features of this Cd-saturation method are: high mnolecular weight Cd-binding proteins are denatured by treatment with acetonitrile (50% final concentration), and excess of Cd is bound to a cation exchange resin (Chelex-100). With this method, MT has been measured, e.g. in liver of control and zinc- or cadmium-treated rats, in human liver and in cultured human fibroblasts down to absolute amounts of 0.1 μg. The Cd-Chelex assay is 10 times more sensitive than the established Cd-heme assay (Dieter et al. 1986) and therefore is particularly suitable to quantify MT in small tissue samples (e.g., liver biopsies of a few milligrams) and in extrahepatic tissues or cell cultures with low MT concentrations.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cadmium ; Cd-saturation Assay ; Chelex ; Metallothionein ; Zinc
Language
english
Publication Year
1990
HGF-reported in Year
0
ISSN (print) / ISBN
0340-5761
e-ISSN
1432-0738
Journal
Archives of Toxicology
Quellenangaben
Volume: 64,
Issue: 3,
Pages: 177-180
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institut für Toxikologie
Scopus ID
0025276084
Erfassungsdatum
1990-12-31