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Unterberger, C. ; Hanson, S.* ; Klingenhoff, A.* ; Oesterle, D.* ; Frankenberger, M. ; Endo, Y.* ; Matsushita, M.* ; Fujita, T.* ; Schwaeble, W.* ; Weiss, E.H.* ; Ziegler-Heitbrock, L. ; Stover, C.*

Stat3 is involved in control of MASP2 gene expression.

Biochem. Biophys. Res. Commun. 364, 1022-1025 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Little is known about determinants regulating expression of Mannan-binding lectin associated serine protease-2 (MASP-2), the effector component of the lectin pathway of complement activation. Comparative bioinformatic analysis of the MASP2 promoter regions in human, mouse, and rat, revealed conservation of two putative Stat binding sites, termed StatA and StatB. Site directed mutagenesis specific for these sites was performed. Transcription activity was decreased 5-fold when StatB site was mutated in the wildtype reporter gene construct. Gel retardation and competition assays demonstrated that proteins contained in the nuclear extract prepared from HepG2 specifically bound double-stranded StatB oligonucleotides. Supershift analysis revealed Stat3 to be the major specific binding protein. We conclude that Stat3 binding is important for MASP2 promoter activity.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Effector component; Lectin pathway; Promoter
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Journal Biochemical and Biophysical Research Communications
Quellenangaben Volume: 364, Issue: 4, Pages: 1022-1025 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Lung Health and Immunity (LHI)