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The isolation of mutants not accumulating poly-β-hydroxybutyric acid.
Arch. Microbiol. 71, 283-294 (1970)
Five mutant strains of Hydrogenomonas H 16 which synthesize poly-β-hydroxybutyric acid either slowly or not at all have been isolated following nitrite and NMG treatment of wild type cells. When grown on a nitrogen deficient agar medium, the colonies of PHB-free cells can be recognized by their diminished retention of the dye sudanblack B. Enrichment procedures for such mutants have been devised employing the 32P-phosphate inactivation technique and sucrose gradient centrifugation. The mutants have been characterized with respect to their growth properties, respiratory control and other properties.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0003-9276
e-ISSN
1432-072X
Journal
Archives of Microbiology
Quellenangaben
Volume: 71,
Issue: 3,
Pages: 283-294
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institut für Biologie