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Ellwart, J.W. ; Brettel, H. ; Kober, L.O.M.

Cell membrane damage by ultrasound at different cell concentrations.

Ultrasound Med. Biol. 14, 43-50 (1988)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Cell membrane damage by ultrasound was studied in human nucleated cells in vitro at various concentrations. Suspensions of human blood cells, cells of a human leukemic cell line (Reh) and mixtures of nucleated cells with erythrocytes were exposed to continuous ultrasound of 782 kHz at a SPTA intensity of 15 W/cm 2. The surviving nucleated cells without membrane damage were counted on the basis of exclusion of ethidiumbromide using flow cytometer. At high cell concentrations as present in whole blood we observed no cell death, whereas below 5 x 10 7 cells/ml most of the granulocytes, stimulated lymphocytes and Reh cells were damaged. The concentration threshold below which cells were damaged seemed not to depend on the size of the cells, rather on the concentration of particles in the suspension. | Cell membrane damage by ultrasound was studied in human nucleated cells in vitro at various concentrations. Suspensions of human blood cells, cells of a human leukemic cell line (Reh) and mixtures of nucleated cells with erythrocytes were exposed to continuous ultrasound of 782 kHz at a SPTA intensity of 15 W/cm**2. The surviving nucleated cells without membrane damage were counted on the basis of exclusion of ethidiumbromide using a flow cytometer. At high cell concentrations as present in whole blood, we observed no cell death, whereas below 5 multiplied by 10**7 cells/ml most of the granulocytes, stimulated lymphocytes and Reh cells were damaged. The concentration threshold below which cells were damaged seemed not to depend on the size of the cells, rather on the concentration of particles in the suspension.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Ultrasound; Cell density; Cell membrane damage; Cavitation; Human leukocytes; Erythrocytes; Dye exclusion; Flow cytometry
ISSN (print) / ISBN 0301-5629
e-ISSN 1879-291X
Journal Ultrasound in Medicine and Biology
Quellenangaben Volume: 14, Issue: 1, Pages: 43-50 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institut für angewandte Optik
Institut für Experimentelle Hämatologie