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Kummer, U. ; Thiel, E.V. ; Doxiadis, I.I.N. ; Eulitz, M. ; Sladoljev, S. ; Thierfelder, S.S.

Tritium radiolabeling of antibodies to high specific activity with N-succinimidyl[2,3-3H]propionate: Use in detecting monoclonal antibodies.

J. Immunol. Methods 42, 367-374 (1981)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
A simple method has been established for tritium labeling immunoglobulins. This label avoids certain disadvantages of 125iodine such as high radiation hazard and requirement for frequent labeling owing to the short half-life of this isotope. High specific activities (range 1.8-9.0 cpm/μg protein) were obtained with no loss of functional activity as demonstrated by passive hemagglutination. The labeled conjugate is especially suitable for detection of activity in hybridoma supernatants by indirect binding assay. Adapted to microtiter plates, the method is suitable for large numbers of samples, e.g. up to 800 supernatants may be examined daily. The utility and advantages of the binding assay with respect to reproductibility, sensitivity, binding kinetics, easy and rapid performance are described.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0022-1759
e-ISSN 1872-7905
Journal Journal of Immunological Methods
Quellenangaben Volume: 42, Issue: 3, Pages: 367-374 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed