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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Haemopoietic inductive capacity of irradiated stromal cell layers in human micro long-term bone marrow cultures.
Cell Prolif. 21, 411-417 (1988)
In a micro long-term bone marrow culture (LTBMC) system the effects of irradiation on confluent stromal cell layers were studied. In order to individually analyse the number of granulocyte-macrophage colony-forming cells (GM-CFC) per LTBMC a miniaturized human GM-CFC assay was established. the normalized GM-CFC numbers in the micro-assay compared well with data by the conventional GM-CFC assay. Pre-formed stromal cell layers were irradiated with doses up to 20 Gy and subsequently recharged with allogeneic bone marrow cells (BMC). Immediately before recharge the BMC were stromal cell-depleted by nylon wool filtration. When stromal cell-depleted BMC were inoculated on empty culture dishes, in vitro haemopoiesis rapidly declined. Sustained GM-CFC production, however, was seen when these cells were used as a second inoculum. It is concluded that irradiation doses of up to 20 Gy do not cause alteration of the haemopoietic inductive capacity of confluent stromal cell layers.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0008-8730
e-ISSN
1365-2184
Journal
Cell Proliferation
Quellenangaben
Volume: 21,
Issue: 6,
Pages: 411-417
Publisher
Blackwell
Publishing Place
Oxford [u.a.] ; Berlin ; Vienna
Reviewing status
Peer reviewed