PuSH - Publication Server of Helmholtz Zentrum München: The role of the spleen in a lactate dehydrogenase mutant mouse (Ldh-1(c)/Ldh-1(c)) with hemolytic anemia.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Datta, T. ; Kremer, J.P. ; Hültner, L. ; Dörmér, P.G.

The role of the spleen in a lactate dehydrogenase mutant mouse (Ldh-1(c)/Ldh-1(c)) with hemolytic anemia.

Exp. Hematol. 16, 281-284 (1988)

DOI

PMC

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

The lactate dehydrogenase mouse mutant Ldh-1(c)/Ldh-1(c) is afflicted with a severe hemolytic anemia associated with extreme reticulocytosis (95%) and splenomegaly. Ninety-one percent of the total body colony-forming units-erythroid (CFU-E) have been quantified in the seven- to ten-times enlarged spleens of the mutant mice. Moreover, the splenic fraction of morphologically recognizable erythroid precursors was 134 times normal. From these data it was apparent that the spleen crucially contributes to the maintenance of steady state erythropoiesis in the mutants. On the other hand, an enhanced sequestration of red blood cells in the enlarged spleen may augment the anemia. Splenectomy experiments were performed with LDH mutant and wild type mice in order to investigate the role of the spleen in this particular hemolytic disease. Following splenectomy, the peripheral blood values and the frequency of femoral stem and progenitor cells were determined, and histological investigations were carried out. The life span of the splenectomized mutants was not shortened, in spite of a very low red blood cell count (25% of the untreated mutant value). Compared to the splenic loss only a moderate increase in bone marrow erythropoiesis was observed, such as a 250% increase of CFU-E. It is concluded that the reduction in red blood cell survival due to splenic sequestration in the mutants is of such a magnitude that it counterbalances a significant portion of splenic erythropoiesis.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0301-472X

e-ISSN 0301-472X

Journal Experimental Hematology

Quellenangaben Volume: 16, Issue: 4, Pages: 281-284

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institut für Experimentelle Hämatologie