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Siebert, W.
;
Glubrecht, H.
Effects of UV- and X-rays on histones in vivo and in vitro.
Radiat. Environ. Biophys.
6
, 19-24 (1969)
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as soon as Postprint is submitted to ZB.
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Histones were isolated by acid extraction from Chinese hamster ovarian tissue culture cells and investigated by means of an amino acid analyzer. Irradiation was performed with a low pressure mercury lamp (2537 A) or with 30 kV-X-ray Bremsstrahlung either of the tissue culture cells before extraction or of the isolated histones in aqueous solution. Both types of radiation affected mainly the aminoacids Tyrosine, Phenylalanine and Histidine in the in vitro experiments. After X-irradiation in vivo comparable doses were without effect. UV-irradiation in vivo led to a decrease of Tyr and Phe like in the in vitro experiments, but in contrast to his finding to an increase of His-concentration. It is tried to give a hypothetic explanation of these results. © 1969 Springer-Verlag.
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0301-634X
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1432-2099
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Radiation and Environmental Biophysics
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Volume: 6,
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Pages: 19-24
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Springer
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