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Bézière, N. ; Lozano, N.* ; Nunes, A. ; Salichs, J. ; Queirós, D. ; Kostarelos, K.* ; Ntziachristos, V.

Dynamic imaging of PEGylated Indocyanine Green (ICG) liposomes within the tumor microenvironment using Multi-Spectral Optoacoustic Tomography (MSOT).

Biomaterials 37, 415-424 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Multispectral optoacoustic tomography (MSOT) is a powerful modality that allows high-resolution imaging of photo-absorbers deep within tissue, beyond the classical depth and resolution limitations of conventional optical imaging. Imaging of intrinsic tissue contrast can be complemented by extrinsically administered gold nanoparticles or fluorescent molecular probes. Instead, we investigated herein generation of re-engineered clinically-used PEGylated liposomes incorporating indocyanine green (LipoICG) as a contrast strategy that combines materials already approved for clinical use, with strong photo-absorbing signal generation available today only from some metallic nanoparticles (e.g. gold nanorods). Using MSOT we confirmed LipoICG as a highly potent optoacoustic agent and resolved tissue accumulation in tumor-bearing animals over time with high-sensitivity and resolution using two tumor models of different vascularisation. We further showcase a paradigm shift in pharmacology studies and nanoparticle investigation, by enabling detailed volumetric optical imaging in vivo through the entire tumor tissue non-invasively, elucidating never before seen spatiotemporal features of optical agent distribution. These results point to LipoICG as a particle with significant advantageous characteristics over gold nanoparticles and organic dyes.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Imaging ; Indocyanine Green ; Liposome ; Optoacoustic ; Photoacoustic ; Tumor
ISSN (print) / ISBN 0142-9612
e-ISSN 1878-5905
Journal Biomaterials
Quellenangaben Volume: 37, Issue: , Pages: 415-424 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)