PuSH - Publication Server of Helmholtz Zentrum München

Washkowitz, A.J.* ; Schall, C.* ; Zhang, K.L.* ; Wurst, W. ; Floß, T. ; Mager, J.* ; Papaioannou, V.E.*

Mga is essential for the survival of pluripotent cells during peri-implantation development.

Development 142, 31-40 (2015)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The maintenance and control of pluripotency is of great interest in stem cell biology. The dual specificity T-box/basic-helix-loop-helix-zipper transcription factor Mga is expressed in the pluripotent cells of the inner cell mass (ICM) and epiblast of the peri-implantation mouse embryo, but its function has not been investigated previously. Here, we use a loss-of-function allele and RNA knockdown to demonstrate that Mga depletion leads to the death of proliferating pluripotent ICM cells in vivo and in vitro, and the death of embryonic stem cells (ESCs) in vitro. Additionally, quiescent pluripotent cells lacking Mga are lost during embryonic diapause. Expression of Odc1, the rate-limiting enzyme in the conversion of ornithine into putrescine in the synthesis of polyamines, is reduced in Mga mutant cells, and the survival of mutant ICM cells as well as ESCs is rescued in culture by the addition of exogenous putrescine. These results suggest a mechanism whereby Mga influences pluripotent cell survival through regulation of the polyamine pool in pluripotent cells of the embryo, whether they are in a proliferative or quiescent state.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Basic-helix-loop-helix-zipper ; Escs ; Mga ; Mouse ; Odc ; Pluripotency ; T-box ; Transcription Factor; Embryonic Stem-cells; Ornithine-decarboxylase Gene; Early Mouse Development; C-myc Expression; Transcription Factor; Self-renewal; Polyamine Metabolism; Ground-state; Max; Network
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Journal Development / Company of Biologists
Quellenangaben Volume: 142, Issue: 1, Pages: 31-40 Article Number: , Supplement: ,
Publisher Company of Biologists
Publishing Place Cambridge
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)