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Open Access Green as soon as Postprint is submitted to ZB.
High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography.
Opt. Lett. 40, 367-370 (2015)
Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Vertex Component Analysis; Stimulated-emission; Microscopy; Gfp; Dronpa; Cells; Limit
ISSN (print) / ISBN
0146-9592
e-ISSN
1539-4794
Journal
Optics Letters
Quellenangaben
Volume: 40,
Issue: 3,
Pages: 367-370
Publisher
Optical Society of America (OSA)
Publishing Place
Washington
Reviewing status
Peer reviewed