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Vinegoni, C.* ; Razansky, D. ; Figueiredo, J.L.* ; Fexon, L.* ; Pivovarov, M.* ; Nahrendorf, M.* ; Ntziachristos, V. ; Weissleder, R.*

Born normalization for fluorescence optical projection tomography for whole heart imaging.

J. Vis. Exp. 28, 1389 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Optical projection tomography is a three-dimensional imaging technique that has been recently introduced as an imaging tool primarily in developmental biology and gene expression studies. The technique renders biological sample optically transparent by first dehydrating them and then placing in a mixture of benzyl alcohol and benzyl benzoate in a 2:1 ratio (BABB or Murray s Clear solution). The technique renders biological samples optically transparent by first dehydrating them in graded ethanol solutions then placing them in a mixture of benzyl alcohol and benzyl benzoate in a 2:1 ratio (BABB or Murray s Clear solution) to clear. After the clearing process the scattering contribution in the sample can be greatly reduced and made almost negligible while the absorption contribution cannot be eliminated completely. When trying to reconstruct the fluorescence distribution within the sample under investigation, this contribution affects the reconstructions and leads, inevitably, to image artifacts and quantification errors.. While absorption could be reduced further with a permanence of weeks or months in the clearing media, this will lead to progressive loss of fluorescence and to an unrealistically long sample processing time. This is true when reconstructing both exogenous contrast agents (molecular contrast agents) as well as endogenous contrast (e.g. reconstructions of genetically expressed fluorescent proteins).
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 1940-087X
e-ISSN 1940-087X
Journal Journal of Visualized Experiments
Quellenangaben Volume: 28, Issue: , Pages: 1389 Article Number: , Supplement: ,
Publisher JoVE
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)