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Open Access Green as soon as Postprint is submitted to ZB.
Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes.
J. Mass Spectrom. 47, 96-104 (2012)
Top-down shotgun lipidomics relies on direct infusion of total lipid extracts into a high-resolution tandem mass spectrometer and implies that individual lipids are recognized by their accurately determined m/z. Lipid ionization efficiency and detection specificity strongly depend on the acquisition polarity, and therefore it is beneficial to analyze lipid mixtures in both positive and negative modes. Hybrid LTQ Orbitrap mass spectrometers are widely applied in top-down lipidomics; however, rapid polarity switching was previously unfeasible because of the severe and immediate degradation of mass accuracy. Here, we report on a method to rapidly acquire high-resolution spectra in both polarity modes with sub-ppm mass accuracy and demonstrate that it not only simplifies and accelerates shotgun lipidomics analyses but also improves the lipidome coverage because more lipid classes and more individual species within each class are recognized. In this way, shotgun analysis of total lipid extracts of human blood plasma enabled to quantify 222 species from 15 major lipid classes within 7 min acquisition cycle.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1076-5174
e-ISSN
1096-9888
Journal
Journal of Mass Spectrometry
Quellenangaben
Volume: 47,
Issue: 1,
Pages: 96-104
Publisher
Wiley
Reviewing status
Peer reviewed
Institute(s)
Institute of Pancreatic Islet Research (IPI)