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Open Access Green as soon as Postprint is submitted to ZB.
In vivo dark-field radiography for early diagnosis and staging of pulmonary emphysema.
Invest. Radiol. 50, 430-435 (2015)
OBJECTIVES: The aim of this study was to evaluate the suitability of in vivo x-ray dark-field radiography for early-stage diagnosis of pulmonary emphysema in mice. Furthermore, we aimed to analyze how the dark-field signal correlates with morphological changes of lung architecture at distinct stages of emphysema. MATERIALS AND METHODS: Female 8- to 10-week-old C57Bl/6N mice were used throughout all experiments. Pulmonary emphysema was induced by orotracheal injection of porcine pancreatic elastase (80-U/kg body weight) (n = 30). Control mice (n = 11) received orotracheal injection of phosphate-buffered saline. To monitor the temporal patterns of emphysema development over time, the mice were imaged 7, 14, or 21 days after the application of elastase or phosphate-buffered saline. X-ray transmission and dark-field images were acquired with a prototype grating-based small-animal scanner. In vivo pulmonary function tests were performed before killing the animals. In addition, lungs were obtained for detailed histopathological analysis, including mean cord length (MCL) quantification as a parameter for the assessment of emphysema. Three blinded readers, all of them experienced radiologists and familiar with dark-field imaging, were asked to grade the severity of emphysema for both dark-field and transmission images. RESULTS: Histopathology and MCL quantification confirmed the introduction of different stages of emphysema, which could be clearly visualized and differentiated on the dark-field radiograms, whereas early stages were not detected on transmission images. The correlation between MCL and dark-field signal intensities (r = 0.85) was significantly higher than the correlation between MCL and transmission signal intensities (r = 0.37). The readers' visual ratings for dark-field images correlated significantly better with MCL (r = 0.85) than visual ratings for transmission images (r = 0.36). Interreader agreement and the diagnostic accuracy of both quantitative and visual assessment were significantly higher for dark-field imaging than those for conventional transmission images. CONCLUSIONS: X-ray dark-field radiography can reliably visualize different stages of emphysema in vivo and demonstrates significantly higher diagnostic accuracy for early stages of emphysema than conventional attenuation-based radiography.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Contrast-computed-tomography; Interferometer; Disease; Quality; Tissue; Signal; Chest; Mice
ISSN (print) / ISBN
0020-9996
e-ISSN
1536-0210
Journal
Investigative Radiology
Quellenangaben
Volume: 50,
Issue: 7,
Pages: 430-435
Publisher
Lippincott Williams & Wilkins
Publishing Place
Hagerstown, Md.
Non-patent literature
Publications
Reviewing status
Peer reviewed