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Open Access Green as soon as Postprint is submitted to ZB.
Identification of early nuclear target genes of plastidial redox signals that trigger the long-term response of Arabidopsis to light quality shifts.
Mol. Plant 8, 1237–1252 (2015)
Natural illumination conditions are highly variable and because of their sessile life style plants are forced to acclimate to them at cellular and molecular level. Changes in light intensity or quality induce changes in the reduction/oxidation (redox) state of the photosynthetic electron chain that act as trigger for compensatory acclimation responses comprising functional and structural adjustments of photosynthesis and metabolism. Such responses include redox-controlled changes in plant gene expression in nucleus and organelles. Here we describe a strategy for the identification of early redox-regulated genes (ERGs) in the nucleus of the model organism Arabidopsis thaliana which significantly respond 30 or 60 min after the generation of a reduction signal in the photosynthetic electron transport chain. By comparing the response of wild-type plants with that of the acclimation mutant stn7 we could specifically identify ERGs. The results reveal a significant impact of chloroplast redox signals on distinct nuclear gene groups including genes for the mitochondrial electron transport chain, tetrapyrrole biosynthesis, carbohydrate metabolism and signalling lipid synthesis. These expression profiles are clearly different from that observed in response to reduction of the photosynthetic electron transport (PET) by high light treatments. The identified ERGs, thus, are unique to redox imbalances in PET and were used for the analysis of potential redox-responsive cis-elements, trans-factors and chromosomal regulatory hot spots. The data identify a novel redox-responsive element and indicate extensive redox control at transcriptional and chromosomal levels that point to an unprecedented impact of redox signals on epigenetic processes.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Acclimation ; Bioenergetics ; Light Signaling ; Photosynthesis ; Physiological ; Redox Control; Photosystem Stoichiometry Adjustment; Mitochondrial Retrograde Regulation; Photosynthetic Electron-transport; Nac Transcription Factor; Mustard Sinapis-alba; Mg-protoporphyrin Ix; State Transitions; Messenger-rna; Higher-plants; Chloroplast Development
ISSN (print) / ISBN
1674-2052
e-ISSN
1752-9867
Journal
Molecular Plant
Quellenangaben
Volume: 8,
Issue: 8,
Pages: 1237–1252
Publisher
Elsevier
Publishing Place
Cambridge
Reviewing status
Peer reviewed