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Open Access Green as soon as Postprint is submitted to ZB.
Microarray analysis of small non-coding RNAs.
Methods Mol. Biol. 1296, 161-171 (2015)
Microarray technology has evolved to efficiently profile the expression of RNAs. However, analysis of small non-coding RNAs (ncRNAs) is challenging due to their short length and highly divergent sequences with large variation in GC content leading to very different hybridization properties. To overcome these challenges, LNA-modified oligonucleotides have been used to enhance and normalize the melting temperature (Tm) of capture probes, which allows sensitive profiling of small ncRNAs regardless of their sequence. Here, we describe the isolation and labeling of small non-coding RNAs, as well as their hybridization to microarrays with LNA-modified oligonucleotide probes using a semi-automated hybridization device.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Rederstorff, M.*
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Small Non-Coding RNAs : Methods and Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1296,
Pages: 161-171
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Diabetes and Cancer (IDC)