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Open Access Green as soon as Postprint is submitted to ZB.
Qualitative and quantitative proteomic analysis of Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Methods Mol. Biol. 1295, 109-115 (2015)
Formalin-fixed, paraffin-embedded (FFPE) tissue has recently gained interest as an alternative to fresh/frozen tissue for retrospective protein biomarker discovery. However, during the formalin fixation proteins undergo degradation and cross-linking, making conventional protein analysis technologies challenging. Cross-linking is even more challenging when quantitative proteome analysis of FFPE tissue is planned. The use of conventional protein labeling technologies on FFPE tissue has turned out to be problematic as the lysine residue labeling targets are frequently blocked by the formalin treatment. We have established a qualitative and quantitative proteomics analysis technique for FFPE tissues that combines label-free proteomic analysis with optimized protein extraction and separation conditions.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Posch, A.*
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Proteomic Profiling : Methods and Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1295,
Pages: 109-115
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Biology (ISB)