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Open Access Green as soon as Postprint is submitted to ZB.
A protocol for the parallel isolation of intact mitochondria from rat liver, kidney, heart, and brain.
Methods Mol. Biol. 1295, 75-86 (2015)
Mitochondria are key organelles for cellular energy production and cell death decisions. Consequently, a plethora of conditions which are toxic to cells are known to directly attack these organelles. However, mitochondria originating from different tissues differ in their sensitivity to toxic insults. Thus, in order to predict the potential organ-specific toxicity of a given drug or pathological condition at the mitochondrial level, test settings are needed that directly compare the responses and vulnerabilities of mitochondria from different organs. As a prerequisite for such test strategies, we provide here a robust, prompt, and easy-to-follow step-by-step protocol to simultaneously isolate functional and intact mitochondria from rat liver, kidney, heart, and brain. This isolation procedure ensures mitochondrial preparations of comparable purity and reproducible quantities which can be subsequently analyzed for organ-specific mitochondrial toxicity.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Posch, A.*
Language
english
Publication Year
2015
HGF-reported in Year
2015
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Proteomic Profiling : Methods and Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1295,
Pages: 75-86
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Molecular Toxicology and Pharmacology (TOX)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
POF-Topic(s)
30203 - Molecular Targets and Therapies
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
G-505200-003
G-505200-001
G-500300-001
G-500390-001
G-505200-001
G-500300-001
G-500390-001
PubMed ID
25820715
Erfassungsdatum
2015-04-01