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Edelmann, F. ; Niedner, A. ; Niessing, D.

ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.

RNA Biol. 12, 233-237 (2015)
Publ. Version/Full Text DOI PMC
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Asymmetric ASH1 mRNA transport during mitosis of budding yeast constitutes one of the best-studied examples of mRNA localization. Recently, 2 studies used in vitro motility assays to prove that motile ASH1 mRNA-transport complexes can be reconstituted entirely from recombinant factors. Both studies, however, differed in their conclusions on whether cargo RNA itself is required for particle assembly and thus activation of directional transport. Here we provide direct evidence that stable complexes do assemble in absence of RNA at physiologic conditions and even at ionic strengths above cellular levels. These results directly confirm the previous notion that the ASH1 transport machinery is not activated by the cargo RNA itself, but rather through protein-protein interactions.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Ash1 Mrna ; Myo4p ; Rna Localization ; She2p ; She3p ; Budding Yeast ; In Vitro Reconstitution ; Mrnp ; Macromolecular Assembly ; Myosin; Class-v Myosin; Messenger-rna; Binding Protein; Saccharomyces-cerevisiae; Translational Repression; Monomeric Myosin; Budding Yeast; Transport; Localization; Myo4p
Language english
Publication Year 2015
HGF-reported in Year 2015
ISSN (print) / ISBN 1547-6286
e-ISSN 1555-8584
Journal RNA Biology
Quellenangaben Volume: 12, Issue: 3, Pages: 233-237 Article Number: , Supplement: ,
Publisher Landes Bioscience
Publishing Place Philadelphia
Reviewing status Peer reviewed
Institute(s) Institute of Structural Biology (STB)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503091-001
DOI 10.1080/15476286.2015.1017217
WOS ID WOS:000352241500001
Scopus ID 84928231212
PubMed ID 25826656
Erfassungsdatum 2015-04-02
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