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Petzold, C.* ; Steinbronn, N.* ; Gereke, M.* ; Strasser, R.H.* ; Sparwasser, T.* ; Bruder, D.* ; Geffers, R.* ; Schallenberg, S.* ; Kretschmer, K.*

Fluorochrome-based definition of naturally occurring Foxp3+ regulatory T cells of intra- and extrathymic origin.

Eur. J. Immunol. 44, 3632-3645 (2014)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Under physiological conditions, studies on the biology of naturally induced Foxp3(+) Treg cells of intra- and extrathymic origin have been hampered by the lack of unambiguous markers to discriminate the mature progeny of such developmental Treg-cell sublineages. Here, we report on experiments in double-transgenic mice, in which red fluorescent protein (RFP) is expressed in all Foxp3(+) Treg cells, whereas Foxp3-dependent GFP expression is exclusively confined to intrathymically induced Foxp3(+) Treg cells. This novel molecular genetic tool enabled us to faithfully track and characterize naturally induced Treg cells of intrathymic (RFP(+) GFP(+) ) and extrathymic (RFP(+) GFP(-) ) origin in otherwise unmanipulated mice. These experiments directly demonstrate that extrathymically induced Treg cells substantially contribute to the overall pool of mature Foxp3(+) Treg cells residing in peripheral lymphoid tissues of steady-state mice. Furthermore, we provide evidence that intra- and extrathymically induced Foxp3(+) Treg cells represent distinct phenotypic and functional sublineages.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Fluorochrome Reporter ; Foxp3 ; Regulatory T Cells ; T-cell Development
Language english
Publication Year 2014
HGF-reported in Year 0
ISSN (print) / ISBN 0014-2980
e-ISSN 1521-4141
Journal European Journal of Immunology
Quellenangaben Volume: 44, Issue: 12, Pages: 3632-3645 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)
PubMed ID 25159127
DOI 10.1002/eji.201444750
Erfassungsdatum 2014-12-31
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