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Open Access Green as soon as Postprint is submitted to ZB.
De novo production of antigen-specific suppressor cells in vivo.
Nat. Protoc. 1, 653-661 (2006)
Foxp3-expressing regulatory T cells (Treg) play an essential role in maintaining tolerance to self antigens and are generated under physiological conditions when developing T cells encounter antigens expressed by thymic epithelial cells. We have addressed the possibility that Treg can be exploited to prevent or even suppress ongoing immune responses to foreign antigens. To this end, one must develop methods that permit the de novo generation of Treg specific for foreign antigens in peripheral lymphoid tissue. This report describes the methodology of generating Treg by delivering minute doses of peptide contained in fusion Abs directed against the DEC-205 endocytic receptor on steady-state dendritic cells. The process, from cloning and production of fusion Abs to antigen-specific Treg induction in vivo, takes approximately 2 months. The results show that delivery of T-cell receptor agonist ligands under subimmunogenic conditions represents a suitable approach for converting naive T cells into Treg.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1754-2189
e-ISSN
1750-2799
Journal
Nature Protocols
Quellenangaben
Volume: 1,
Issue: 2,
Pages: 653-661
Publisher
Nature Publishing Group
Reviewing status
Peer reviewed
Institute(s)
Institute of Pancreatic Islet Research (IPI)